PCR amplification introduces errors into mononucleotide and dinucleotide repeat sequences
نویسندگان
چکیده
منابع مشابه
PCR amplification introduces errors into mononucleotide and dinucleotide repeat sequences.
The polymerase chain reaction (PCR) is used universally for accurate exponential amplification of DNA. We describe a high error rate at mononucleotide and dinucleotide repeat sequence motifs. Subcloning of PCR products allowed sequence analysis of individual DNA molecules from the product pool and revealed that: (1) monothymidine repeats longer than 11 bp are amplified with decreasing accuracy,...
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ژورنال
عنوان ژورنال: Molecular Pathology
سال: 2001
ISSN: 1366-8714
DOI: 10.1136/mp.54.5.351